Data for "Transvitreal retinochoroidal biopsies of primary uveal melanoma reveal an association of low HLA class I and high NK cell abundance in low-risk disease"

This data was collected for research purposes. The findings and results have been published in a manuscript called "Transvitreal retinochoroidal biopsies of primary uveal melanoma reveal an association of low HLA class I and high NK cell abundance in low-risk disease" In this study, transvitreal retinochoroidal biopsies were taken from a total of 41 patients with primary uveal melanoma as diagnosis. These biopsies were used for the analysis of immune cells by flow cytometry. Three flow cytometry panels were implemented to study tumor infiltrating lymphocytes (T cells, NK cells and B cells), including their activation/exhaustion markers, as well as HLA class I, HLA class II and PD-L1 and PD-L2 expression on tumor cells and T cells. Additionally, on archived tissue samples from a total of 13 patients that underwent enucleation, we performed multiplex immunohistochemistry to study lymphocytes in the different components of the eye (tumor, choroid, iris and ciliary body). From multiplex immunohistochemistry, densities can also be calculated, rather than just percentages, thereby indicating absolute infiltration. For all included patients, relevant clinical data was collected as well. This includes follow-up of tumor recurrence, tumor size measurements, and genetic risk factors.