Effect of hyaluronan in collagen biomaterials on human macrophages and fibroblasts in vitro

This data collection contains research data underlying the results of a scientific research article. Below is a short explanation of the experiments and the content of the collection. Background: Deep skin wounds in adults typically heal with scar formation, unlike fetal wounds, which regenerate without scarring. Hyaluronan (HA), a major component of the fetal extracellular matrix, is thought to contribute to this regenerative capacity. Goal of the research: To investigate whether incorporating HA into type I collagen scaffolds can improve wound healing outcomes. We examined how these scaffolds influence immune responses and fibrotic activity in different human primary skin cell types. The scaffolds were characterized for HA content, morphology, denaturation temperature, and free amine content. The biological effects were evaluated by measuring cell surface markers and cytokine expression in macrophages. In parallel, protein and mRNA expression levels were analyzed in fetal, adult, and eschar-derived fibroblasts. This collection contains images for the scaffold analysis as well as data from its biochemical characterization such as Differential scanning calorimetry, hyaluronan content and amine group content. Additionally, in vitro data with macrophages includes FACS and ELISA analysis while fibroblast samples include western blotting, PCR and histology images.