Galectin‐9 interacts with Vamp‐3 to regulate cytokine secretion in dendritic cells

This dataset was generated as part of a research project investigating the intracellular mechanisms that regulate cytokine secretion in human dendritic cells during immune activation, with a particular focus on the role of galectin-9 and its interaction with vesicle trafficking proteins. Dendritic cells play a central role in initiating immune responses, and understanding how cytokine-containing vesicles are transported and secreted is critical for elucidating immune regulation and dysregulation in inflammatory and infectious diseases. The study combines cell biology, imaging, flow cytometry, biochemical assays, and proteomics approaches to characterize molecular interactions and trafficking pathways involved in dendritic cell activation. The dataset includes raw microscopy, live-cell imaging, flow cytometry, ELISA, and western blot data generated to support the conclusions of the manuscript. Microscopy data are provided in .czi or .lif formats (containing all images in a single compressed file), live-cell microscopy data are provided in .avi format, flow cytometry data are provided in .fcs format, ELISA data are provided in .mpl and .xls formats, and western blots are uploaded in their original raw image format (.tif). Sample names are self-explanatory in each file name; when additional clarification is required, accompanying metadata Excel files describe sample identity, experimental conditions, and data formats. Quantification and downstream analyses are included where applicable as Excel spreadsheets or GraphPad Prism files. Mass spectrometry data associated with Figure 6A are deposited separately in the PRIDE repository. These raw data enable reuse for secondary analyses, methodological comparisons, and integration with related datasets in immunology, cell biology, and vesicle trafficking research.